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IgG Test Supplies |
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DVM™ RAPID TEST
NOW, A FAST, SIMPLE, PRECISE, LOW COST PORTABLE TEST SYSTEM
FOR
IgG/SPERM CONCENTRATION!
DVM Rapid Test Accurately Measures
IgG/Sperm Counts in Only 10 Minutes in the Laboratory or Field |
| The DVM Rapid Test™ from Value Diagnostics is a cost-effective, portable, highly accurate point-of-care instrument that comes
in a variety of kit configurations and wavelength settings. It includes an instrument package designed to give a quantitative
measurement of IgG in serum, plasma and colostrum or sperm concentration by turbidimetric assay. This test system can be used
either in the laboratory or on-site. |
| DVM Rapid Test™ Kits for IgG/Sperm Counts
Point-of-Care Measurement.
Both Instrument Kit and Reagent Kits Completely Turn-key.
Turbidimetric Immunoassay System for Serum and Colostrum IgG.
Serum or Plasma IgG Validated for Bovine, Camelid, Equine and Deer.
Colostrum IgG Validated for Bovine and Equine.
Sperm Concentrations Validated for Bovine, Canine, Equine and Porcine.
Rapid, Simple, Easy-to-Learn and Use.
Accurate.
Economical. |
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The DVM Rapid Test has been a lifesaver ... literally! To have the peace of mind to quickly run an accurate IgG test right there at the farm is just terrific. It gives us the ability to begin treating a cria within an hour of drawing the blood for the test if that needs to happen. Or within an hour, we can kick back in the rockers and watch the cria run in the field knowing that she's gotten Total Passive Transfer and doesn't need a thing.
The DVM Rapid Test is much easier to use than other IgG testing products we've purchased. Those other tests were run at the vet's office and we had to wait 24 hours for results. Everyone who knows me knows how impatient I am ... especially when it comes to the health of one of my alpacas. So the fact that Dennis and I can run the test at our farm and get fast, accurate results is great.
The DVM Rapid Test is simple to use with just a few steps. Our old IgG test kit required careful handling of the plate. And getting results meant waiting 24 hours then doing measurements and calculations that I haven't done since my Trigonometry class in college! With the DVM Rapid Test, the preparation of the tube takes maybe a half hour. Then to get the results, we just pop the test tube in the machine and it does the work for us.
Investing in the DVM Rapid Test was a smart business move for our farm. While the initial set up may seem expensive, when you consider the value of just one cria ... it's easy to justify the cost. Plus, we are able to provide our clients with a value added service not available at all farms. When dams boarding at our place deliver, we can let our clients know right away how their new cria is doing.
We highly recommend the DVM Rapid Test!
Dennis & Monica
a Suri Farm, ltd.
Fine Peruvian Alpacas in the Heart of Pennsylvania Dutch Country
Dennis L. Balbac & Monica E. Kline
1864 Mount Zion Road
Myerstown, PA 17067-3307
Ph: 717-514-0022
www.asurifarm.com |
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Fast IgG Test Can Help Save Newborn Ruminants
IgG is an antibody protein whose purpose is to provide immunity from
foreign material. The measurement of serum/plasma IgG levels in newborn
ruminants around 24 hours after birth indicates how successfully immunity
has transferred from the mother to the newborn through ingestion of her
colostrum. Newborn calves have little or no circulating IgG and depend
on colostrum to fight infectious agents – this passive transfer of immunity
fails in up to 10 percent of all newborn ruminants, putting these animals
at a high risk of illness or death.
Rapidly identifying newborns with inadequate immunity is critical in
determining the need for IgG supplementation and the level of treatment
that’s necessary. The longer treatment is delayed, the more at risk the
newborn becomes. Greater than 800 mg of IgG per 100ml of plasma
(mg/dl) is considered an adequate level of immunity, while levels below
400 mg indicate Failure of Passive Transfer (FPT). Newborns with IgG
levels between 400 mg and 800 mg may also be at risk. Post treatment
testing for IgG levels is recommended to confirm that a treatment has
been successful. |
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Introducing Greater Speed and Accuracy
Common ways to measure IgG concentration – such as the radial immunodiffusion (RFID) test – require long diffusion times of 24 to 72 hours and are labor intensive. Refractometry, total protein measurements and sodium sulfite turbidity testing are highly variable and lack specificity.
A manual turbidimetric immunoassay that is a modification of an automated assay procedure introduced in 1997 reduces analysis time to 10 minutes, and can be performed on-site using an inexpensive fixed wavelength photometer.
The Science – How the IgG Test Works
Measurement of immune complexes by Spectrophotometry (a.k.a. Turbidimetry) is unlike classical biochemistry where the reactants are clear and endpoints are expressed as absorbances. That’s because the behavior of light differs for solutions containing suspensions or particulates. Such insoluble immune complexes are formed as a reaction between antigens (IgG in the serum/plasma) and antibodies (Goat Anti-IgG) that takes place in a polyethylene glycol based medium.
IgG Testing Manuals
DVM Rapid Test - Operators Manual
Camelid Serum IgG TIA Product
Serum and Colostrum IgG Pictorial Procedure
Clinical Evaluation of the DVM Rapid Test
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The Serum and Colostrum IgG Pictorial Procedure gives step by step
instructions. |
Sperm Counts, Too
The DVM Rapid Test can be used to measure sperm concentration, using an optional Sperm Concentration Reagent validated for bovine, canine, equine and porcine semen testing. The procedure is similar to IgG testing. It uses the same turbidimetric principles to measure changes in turbidity in the test solution related to sperm concentration in a linear relationship over a specific range of sperm cell concentration. |
DVM Rapid Test™ Instrument Packages:
Instrument packages are designed to meet individual needs. All instrument packages are turn-key and come with micropipettors, tips, a timer, test tube rack and quality control standards. Easy to use and requiring no special training, the system is completely portable and can be used in the lab and in the field.
IgG Test Kits:
Turn-key kits include pre-loaded test tubes, dilution tubes (colostrum kits), a reagent blank tube and an IgG control (for optional quality control). IgG test kits are available with 10 or 20 tests per kit.
Sperm Concentration Kits:
Turn-key kits that include sperm concentration reagent, test tubes and caps. Sperm concentration kits are available with 25, 125 or 1000 tests per kit.
Save Money by Testing for IgG On the Farm The DVM Rapid Test™ system has been shown to cut the cost of testing for serum IgG levels in new born ruminants by as much as 75 percent over the cost of paying a reference lab to perform the same test and shipping. The system will typically pay for itself in one year or less.
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The DVM Rapid Test Takes These Steps:
Light of a determined wavelength is allowed to pass through the reaction solution containing only the
antibodies (Reagent Blank).
This initial absorbance of light is measured and set as a zero point (Blanking).
The test sample containing the antigen (analyte) is added to the antibody reagent solution, mixed and allowed to react.
When a single molecule binds to at least two corresponding binding sites on antigen particles, an agglutination takes place.
The agglutinating particles aggregate and form immune complexes that increase in size, resulting in an increase in turbidity of the solution.
This decreases the quantity of the incident light that is transmitted (%T).
The DVM Rapid Test photometer reads this decreased transmitted light (%T).
The decrease in %T is related to the concentration of antigen (IgG) in a linear relationship over a specific range of
analyte concentration (Assay range).
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Safety Lancets & Micro Tubes
 Single-use
lancets feature recessed needles or blades for precise placement
before use. Simply position the lancet and push the button.
Automatic and permanent needle/blade retraction prevents
accidental sticks after puncture. Sterile. |
Designed for the collection, transport, and processing of skin puncture blood. The integrated collection scoop, along with a wider diameter tube, allow for ease of collection and improved mixing. To simplify additive and sample identification, tubes are color-coded in a manner similar to evacuated tubes. Volume level lines, printed on tubes, ensure proper blood-to-anticoagulant ratio.
The “twist-assist” BD Microgard* closure is ready-to-use and reduces blood splatter upon removal. |
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IgG Tips for use
The DVM Rapid Test looks complicated and imposing however it is a relatively simple process.
While the DVM Rapid Test was designed for & is used by vets, I learned very easily how to use the DVM Rapid Test with no medical or technical background.
The steps are simple:
1) Collect blood from the cria (more on this later)
2) Spin the blood in a centrifuge to separate the serum
3) Draw off a very small amount of serum (clear part of the blood) & mix with a prepared reagent
4) After 10 minutes place the tube containing the serum & reagent mixture in the DVM Rapid Test instrument
5) The DVM Rapid Test measures the cloudiness of the mixture and provides a reading
6) The reading is them compared to a chart to determine the IgG level
If you supplement orally with cow or goat colostrum we have reagents that will measure the resulting IgG
The reagents are sold separately from the instrument (DVM Rapid Test) itself.
The reagents are sold in packages 10 and 20 tests and have a shelf life of 2 years.
Be sure to order reagents when purchasing the DVM Rapid Test.
The DVM Rapid Test is easier to use than the alternatives.
There is much less chance of invalidating or ruining the test components in the DVM Rapid Test than the typical alternative as well.
Blood collection can be done a number of ways:
Using a needle & syringe to draw from the jugular in the neck or from a vein in the ear or tail.
NEW: We now offer a less imposing method of collection using a lancet (like a heel stick done on babies or finger prick when giving blood) and a mini test tube called a microtainer. The lancet is used to create a tiny incision under the tail and the droplets of blood are collected in the microtainer. The microtainer is placed in the centrifuge and spun like any other test tube to separate the serum.
The DVM Rapid Test instrument includes a sensor that checks for minimum power to operate properly. If this sensor detects insufficient power the battery dead message will come up in the display during start-up and then shut off the instrument. So as long as the instrument stays on during operation there should be no affect on accuracy as the battery weakens.
Experience in the past has indicated that one should be able to perform several thousand measurements on one set of batteries.
If one is short on a sample (less than 10ul) then you will get a value that is lower than the actual value and would manifest as a higher %T reading. (Remember there is an inverse relationship between %T and Concentration). This is typically caused by getting an small air bubble in the sample in the pipette tip; this is why the instructions state that one should visually check the sample in the tip to check for air bubbles and if an air bubble is observed the tip should be discarded and the serum/plasma sample should be reacquired using a new tip. Also note that not all pipette tips work the same with the micro-pipettes that we sell. The pipette tips we sell are the best fit at the best price.
If one over samples (greater than 10ul) then you will get a value that is higher than the actual value and would manifest as a lower %T reading. This is typically caused by getting extra sample on the outside of the pipette tip; this is why the instructions state that one should touch the tip to the edge of the sample container after acquiring the sample to allow any excess sample to wick off.
If the instrument is not responding properly, which can be determined by use of the turbidity standards, then an erroneous reading may result. Typically being a higher %T and a falsely low concentration.
Please call me with any questions or for advice. 866-999-AVA1 (2821)
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