 |
Check out what customers have to say |
 |
Quick Use Guide: IgG Assay [Plasma] using the DVM Rapid Test™ NOW, A FAST, SIMPLE, PRECISE, LOW COST PORTABLE TEST SYSTEM FOR IgG Testing DVM Rapid Test Accurately Measures IgG in Only 10 Minutes in the Laboratory or Field |
| The DVM Rapid Test™ from Value Diagnostics is a cost-effective, portable, highly accurate point-of-care instrument that comes in a variety of kit configurations and wavelength settings. It includes an instrument package designed to give a quantitative measurement of IgG in serum, plasma and colostrum concentration by turbidimetric assay. This test system can be used either in the laboratory or on-site. |
|
|
||
IgG is an antibody protein whose purpose is to provide immunity from foreign material. The measurement of serum/plasma IgG levels in newborn ruminants around 24 hours after birth indicates how successfully immunity has transferred from the mother to the newborn through ingestion of her colostrum. Newborn calves have little or no circulating IgG and depend on colostrum to fight infectious agents – this passive transfer of immunity fails in up to 10 percent of all newborn ruminants, putting these animals at a high risk of illness or death. Rapidly identifying newborns with inadequate immunity is critical in determining the need for IgG supplementation and the level of treatment that’s necessary. The longer treatment is delayed, the more at risk the newborn becomes. Greater than 800 mg of IgG per 100ml of plasma (mg/dl) is considered an adequate level of immunity, while levels below 400 mg indicate Failure of Passive Transfer (FPT). Newborns with IgG levels between 400 mg and 800 mg may also be at risk. Post treatment testing for IgG levels is recommended to confirm that a treatment has been successful. |
|
Introducing Greater Speed and Accuracy Common ways to measure IgG concentration – such as the radial immunodiffusion (RFID) test – require long diffusion times of 24 to 72 hours and are labor intensive. Refractometry, total protein measurements and sodium sulfite turbidity testing are highly variable and lack specificity. A manual turbidimetric immunoassay that is a modification of an automated assay procedure introduced in 1997 reduces analysis time to 10 minutes, and can be performed on-site using an inexpensive fixed wavelength photometer. The Science – How the IgG Test Works Measurement of immune complexes by Spectrophotometry (a.k.a. Turbidimetry) is unlike classical biochemistry where the reactants are clear and endpoints are expressed as absorbances. That’s because the behavior of light differs for solutions containing suspensions or particulates. Such insoluble immune complexes are formed as a reaction between antigens (IgG in the serum/plasma) and antibodies (Goat Anti-IgG) that takes place in a polyethylene glycol based medium. IgG Testing Manuals |
 |
|
DVM Rapid Test™ Instrument Packages: Instrument packages are designed to meet individual needs. All instrument packages are turn-key and come with micropipettors, tips, a timer, test tube .gif) rack and quality control standards. Easy to use and requiring no special training, the system is completely portable and can be used in the lab and in the field.IgG Test Kits: Turn-key kits include pre-loaded test tubes, dilution tubes (colostrum kits), a reagent blank tube and an IgG control (for optional quality control). IgG test kits are available with 10 or 20 tests per kit. Save Money by Testing for IgG On the Farm The DVM Rapid Test™ system has been shown to cut the cost of testing for serum IgG levels in new born ruminants by as much as 75 percent over the cost of paying a reference lab to perform the same test and shipping. The system will typically pay for itself in one year or less. |
| IgG Starter Kits
|
||||||||||||
|
|
|||||||||||
|
|
| IgG Supplies | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
Drawing Blood for an IgG test From A Cria's Tail Drawing Blood for an IgG test From A Cria's Neck |
Drawing Blood for an IgG test From A Cria's Leg Performing an IgG Test |
The DVM Rapid Test Takes These Steps: antibodies (Reagent Blank). analyte concentration (Assay range). |
 Single-use
lancets feature recessed needles or blades for precise placement
before use. Simply position the lancet and push the button.
Automatic and permanent needle/blade retraction prevents
accidental sticks after puncture. Sterile. |
|
 Designed for the collection, transport, and processing of skin puncture blood. The integrated collection scoop, along with a wider diameter tube, allow for ease of collection and improved mixing. To simplify additive and sample identification, tubes are color-coded in a manner similar to evacuated tubes. Volume level lines, printed on tubes, ensure proper blood-to-anticoagulant ratio.The “twist-assist” BD Microgard* closure is ready-to-use and reduces blood splatter upon removal. |
|
The DVM Rapid Test looks complicated and imposing however it is a relatively simple process. While the DVM Rapid Test was designed for & is used by vets, I learned very easily how to use the DVM Rapid Test with no medical or technical background. The steps are simple: 1) Collect blood from the cria (more on this later) 2) Spin the blood in a centrifuge at 3000 rpm for 10 minutes to separate the serum 3) Draw off a very small amount of serum (clear part of the blood) & mix with a prepared reagent 4) After 10 minutes place the tube containing the serum & reagent mixture in the DVM Rapid Test instrument 5) The DVM Rapid Test measures the cloudiness of the mixture and provides a reading 6) The reading is them compared to a chart to determine the IgG level If you supplement orally with cow or goat colostrum we have reagents that will measure the resulting IgG The reagents are sold separately from the instrument (DVM Rapid Test) itself. The reagents are sold in packages 10 and 20 tests and have a shelf life of 2 years. Be sure to order reagents when purchasing the DVM Rapid Test. The DVM Rapid Test is easier to use than the alternatives. There is much less chance of invalidating or ruining the test components in the DVM Rapid Test than the typical alternative as well. Blood collection can be done a number of ways: Using a needle & syringe to draw from the jugular in the neck or from a vein in the ear or tail. NEW: We now offer a less imposing method of collection using a lancet (like a heel stick done on babies or finger prick when giving blood) and a mini test tube called a microtainer. The lancet is used to create a tiny incision under the tail and the droplets of blood are collected in the microtainer. The microtainer is placed in the centrifuge and spun like any other test tube to separate the serum. The DVM Rapid Test instrument includes a sensor that checks for minimum power to operate properly. If this sensor detects insufficient power the battery dead message will come up in the display during start-up and then shut off the instrument. So as long as the instrument stays on during operation there should be no affect on accuracy as the battery weakens. Experience in the past has indicated that one should be able to perform several thousand measurements on one set of batteries. If one is short on a sample (less than 10ul) then you will get a value that is lower than the actual value and would manifest as a higher %T reading. (Remember there is an inverse relationship between %T and Concentration). This is typically caused by getting an small air bubble in the sample in the pipette tip; this is why the instructions state that one should visually check the sample in the tip to check for air bubbles and if an air bubble is observed the tip should be discarded and the serum/plasma sample should be reacquired using a new tip. Also note that not all pipette tips work the same with the micro-pipettes that we sell. The pipette tips we sell are the best fit at the best price. If one over samples (greater than 10ul) then you will get a value that is higher than the actual value and would manifest as a lower %T reading. This is typically caused by getting extra sample on the outside of the pipette tip; this is why the instructions state that one should touch the tip to the edge of the sample container after acquiring the sample to allow any excess sample to wick off. If the instrument is not responding properly then an erroneous reading may result. This can be determined by use of the turbidity standards. Typically you will find a higher %T and a falsely low concentration. We suggest that you store reagents in the refrigerator (we do too) and we ship with cold packs to avoid high spikes in temps which can affect the reagents. Reagents have been tested at and by the manufacturer after storing at room temp for 2 yrs and are 100% effective. Since most of us don’t have controlled temp rooms the refrigerator is the best alternative for a controlled temp setting to avoid the heat spikes of a boiling hot summer day. If you believe that your reagents have been compromised you can test them by using the control that came with the reagents and see if you get a good reading. This will use one test but you’ll know if all others are OK. Please call us with any questions or for advice. 866-999-AVA1 (2821)  Back to Top |
|
Q - If a breeder does their own IgG test with our DVM Rapid Test will that be satisfactory for insurance purposes? A: Yes, that is fine if you do your own testing!! thanks, Stacy Miller Wilkins Livestock Insurers, Inc. (402) 759-4901 Q - We used the “800” standard in the DVM Rapid Test and came up with a 1600 IgG, Is this correct? A: The Turbidity Standards have NOTHING to do with IgG testing. The Turbidity Standards, as stipulated in Section 4.5 of the operators manual, are for the purpose of checking that the instrument is operating properly. The results are expressed only as %T readings. The acceptable range of %T values for each standard are listed in the DVM Rapid Test QC Log ( last page of the operators manual). The %T values obtained in this procedure are not associated with any test charts or IgG values found in the data manual. Q - If we use the camelid serum packed with the reagents, should the result come within the range shown on the enclosed paperwork? A: The IgG Control Serum included with every kit should be treated in the same manner as a test sample. Follow the test procedure used for unknowns. These controls are recommended as a means of ensuring that all tests are being run properly. It is recommended that users run the quality control sample provided with the test kits at least once with each new test kit to insure that the test is being performed properly and that patient values are accurate. Use the test log to record results. Note: A range of acceptable concentrations is listed at the bottom of the IgG Control product insert include in every test kit. As long as the Control value(s) fall with-in the range of listed values the patient values should be determined accurate. If the Control value(s) are found to be out of range repeat testing of the control is recommended. If the results are still out of range call Tech Services at 715-778-5854. |