Quick Use Guide: IgG Assay [Plasma] using the DVM Rapid Test™
NOW, A FAST, SIMPLE, PRECISE, LOW COST PORTABLE TEST SYSTEM
FOR IgG Testing
DVM Rapid Test Accurately Measures IgG in Only 10 Minutes in the Laboratory or Field
The DVM Rapid Test™ from Value Diagnostics is a cost-effective, portable, highly accurate point-of-care instrument that comes in a variety of kit configurations and wavelength settings. It includes an instrument package designed to give a quantitative measurement of IgG in serum, plasma and colostrum concentration by turbidimetric assay. This test system can be used either in the laboratory or on-site.
DVM Rapid Test™ Kits for IgG
- Point-of-Care Measurement.
- Both Instrument Kit and Reagent Kits Completely Turn-key.
- Turbidimetric Immunoassay System for Serum and Colostrum IgG.
- Serum or Plasma IgG Validated for Bovine, Camelid, and Equine.
- Colostrum IgG Validated for Bovine and Equine.
- Rapid, Simple, Easy-to-Learn and Use.
Fast IgG Test Can Help Save Newborn Ruminants
IgG is an antibody protein whose purpose is to provide immunity from foreign material. The measurement of serum/plasma IgG levels in newborn ruminants around 24 hours after birth indicates how successfully immunity has transferred from the mother to the newborn through ingestion of her colostrum. Newborn calves have little or no circulating IgG and depend on colostrum to fight infectious agents – this passive transfer of immunity fails in up to 10 percent of all newborn ruminants, putting these animals at a high risk of illness or death.
Rapidly identifying newborns with inadequate immunity is critical in determining the need for IgG supplementation and the level of treatment that’s necessary. The longer treatment is delayed, the more at risk the newborn becomes. Greater than 800 mg of IgG per 100ml of plasma (mg/dl) is considered an adequate level of immunity, while levels below 400 mg indicate Failure of Passive Transfer (FPT). Newborns with IgG levels between 400 mg and 800 mg may also be at risk. Post treatment testing for IgG levels is recommended to confirm that a treatment has been successful.
The DVM Rapid Test is much easier to use than other IgG testing products we’ve purchased. Those other tests were run at the vet’s office and we had to wait 24 hours for results. Everyone who knows me knows how impatient I am … especially when it comes to the health of one of my alpacas. So the fact that Dennis and I can run the test at our farm and get fast, accurate results is great.
The DVM Rapid Test II is simple to use with just a few steps. Our old IgG test kit required careful handling of the plate. And getting results meant waiting 24 hours then doing measurements and calculations that I haven’t done since my Trigonometry class in college! With the DVM Rapid Test II, the preparation of the tube takes maybe a half hour. Then to get the results, we just pop the test tube in the machine and it does the work for us.
Investing in the DVM Rapid Test II was a smart business move for our farm. While the initial set up may seem expensive, when you consider the value of just one cria … it’s easy to justify the cost. Plus, we are able to provide our clients with a value added service not available at all farms. When dams boarding at our place deliver, we can let our clients know right away how their new cria is doing.
We highly recommend the DVM Rapid Test II!
Dennis & Monica
a Suri Farm, ltd.
Fine Peruvian Alpacas in the Heart of Pennsylvania Dutch Country
Dennis L. Balbac & Monica E. Kline
1864 Mount Zion Road
Myerstown, PA 17067-3307
Common ways to measure IgG concentration – such as the radial immunodiffusion (RFID) test – require long diffusion times of 24 to 72 hours and are labor intensive. Refractometry, total protein measurements and sodium sulfite turbidity testing are highly variable and lack specificity.
A manual turbidimetric immunoassay that is a modification of an automated assay procedure introduced in 1997 reduces analysis time to 10 minutes, and can be performed on-site using an inexpensive fixed wavelength photometer.
The Science – How the IgG Test Works
Measurement of immune complexes by Spectrophotometry (a.k.a. Turbidimetry) is unlike classical biochemistry where the reactants are clear and endpoints are expressed as absorbances. That’s because the behavior of light differs for solutions containing suspensions or particulates. Such insoluble immune complexes are formed as a reaction between antigens (IgG in the serum/plasma) and antibodies (Goat Anti-IgG) that takes place in a polyethylene glycol based medium.
IgG Testing Manuals
DVM Rapid Test II™ Instrument Packages:
Instrument packages are designed to meet individual needs. All instrument packages are turn-key and come with micropipettors, tips, a timer, test tube rack and quality control standards. Easy to use and requiring no special training, the system is completely portable and can be used in the lab and in the field.
IgG Test Kits:
Turn-key kits include pre-loaded test tubes, dilution tubes (colostrum kits), a reagent blank tube and an IgG control (for optional quality control). IgG test kits are available with 10 or 20 tests per kit.
Save Money by Testing for IgG On the Farm
The DVM Rapid Test II™ system has been shown to cut the cost of testing for serum IgG levels in new born ruminants by as much as 75 percent over the cost of paying a reference lab to perform the same test and shipping. The system will typically pay for itself in one year or less.