IgG Test Kits

DVM Rapid Test™ Kits for IgG

• Point-of-Care Measurement.
• Both Instrument Kit and Reagent Kits Completely Turn-key.
• Turbidimetric Immunoassay System for Serum and Colostrum IgG.
• Serum or Plasma IgG Validated for Bovine, Camelid, and Equine.
• Colostrum IgG Validated for Bovine and Equine.
• Rapid, Simple, Easy-to-Learn and Use.
• Accurate.
• Economical.

Fast IgG Test Can Help Save Newborn Ruminants
IgG is an antibody protein whose purpose is to provide immunity from foreign material. The measurement of serum/plasma IgG levels in newborn ruminants around 24 hours after birth indicates how successfully immunity has transferred from the mother to the newborn through ingestion of her colostrum. Newborn calves have little or no circulating IgG and depend on colostrum to fight infectious agents – this passive transfer of immunity fails in up to 10 percent of all newborn ruminants, putting these animals at a high risk of illness or death.

Rapidly identifying newborns with inadequate immunity is critical in determining the need for IgG supplementation and the level of treatment that’s necessary. The longer treatment is delayed, the more at risk the newborn becomes. Greater than 800 mg of IgG per 100ml of plasma (mg/dl) is considered an adequate level of immunity, while levels below 400 mg indicate Failure of Passive Transfer (FPT). Newborns with IgG levels between 400 mg and 800 mg may also be at risk. Post treatment testing for IgG levels is recommended to confirm that a treatment has been successful.

The DVM Rapid Test has been a lifesaver … literally! To have the peace of mind to quickly run an accurate IgG test right there at the farm is just terrific. It gives us the ability to begin treating a cria within an hour of drawing the blood for the test if that needs to happen. Or within an hour, we can kick back in the rockers and watch the cria run in the field knowing that she’s gotten Total Passive Transfer and doesn’t need a thing.

The DVM Rapid Test is much easier to use than other IgG testing products we’ve purchased. Those other tests were run at the vet’s office and we had to wait 24 hours for results. Everyone who knows me knows how impatient I am … especially when it comes to the health of one of my alpacas. So the fact that Dennis and I can run the test at our farm and get fast, accurate results is great.

The DVM Rapid Test II is simple to use with just a few steps. Our old IgG test kit required careful handling of the plate. And getting results meant waiting 24 hours then doing measurements and calculations that I haven’t done since my Trigonometry class in college! With the DVM Rapid Test II, the preparation of the tube takes maybe a half hour. Then to get the results, we just pop the test tube in the machine and it does the work for us.

Investing in the DVM Rapid Test II was a smart business move for our farm. While the initial set up may seem expensive, when you consider the value of just one cria … it’s easy to justify the cost. Plus, we are able to provide our clients with a value added service not available at all farms. When dams boarding at our place deliver, we can let our clients know right away how their new cria is doing.

We highly recommend the DVM Rapid Test II!
Dennis & Monica
a Suri Farm, ltd.
Fine Peruvian Alpacas in the Heart of Pennsylvania Dutch Country
Dennis L. Balbac & Monica E. Kline
1864 Mount Zion Road
Myerstown, PA 17067-3307
Ph: 717-514-0022
www.asurifarm.com

Introducing Greater Speed and Accuracy
Common ways to measure IgG concentration – such as the radial immunodiffusion (RFID) test – require long diffusion times of 24 to 72 hours and are labor intensive. Refractometry, total protein measurements and sodium sulfite turbidity testing are highly variable and lack specificity.

A manual turbidimetric immunoassay that is a modification of an automated assay procedure introduced in 1997 reduces analysis time to 10 minutes, and can be performed on-site using an inexpensive fixed wavelength photometer.

The Science – How the IgG Test Works
Measurement of immune complexes by Spectrophotometry (a.k.a. Turbidimetry) is unlike classical biochemistry where the reactants are clear and endpoints are expressed as absorbances. That’s because the behavior of light differs for solutions containing suspensions or particulates. Such insoluble immune complexes are formed as a reaction between antigens (IgG in the serum/plasma) and antibodies (Goat Anti-IgG) that takes place in a polyethylene glycol based medium.

IgG Testing Manuals

• Camelid Serum IgG TIA Product
• Quick Use Guide: IgG Assay [Serum/Plasma] using the DVM Rapid Test™
• Clinical Evaluation of the DVM Rapid Test II